Glucocorticoids impair fetal beta-cell development in rats

Am J Physiol Endocrinol Metab. 2001 Sep;281(3):E592-9. doi: 10.1152/ajpendo.2001.281.3.E592.

Abstract

In rats, poor fetal growth due to maternal food restriction during pregnancy is associated with decreased beta-cell mass at birth and glucose intolerance in adulthood. Overexposure to glucocorticoids in utero can induce intrauterine growth retardation in humans and animals and subsequent glucose intolerance in rodents. The aims of this study were to investigate whether glucocorticoid overexposure mediates the effect of undernutrition on beta-cell mass and to study their potential role in normally nourished rats. Undernutrition significantly increased maternal and fetal corticosterone levels. Twenty-one-day-old fetuses with undernutrition showed growth retardation and decreased pancreatic insulin content; adrenalectomy and subcutaneous corticosterone implants in their dams prevented the maternal corticosterone increase and restored fetal beta-cell mass. In fetuses with normal nutrition, fetal corticosterone levels were negatively correlated to fetal weight and insulin content; fetal beta-cell mass increased from 355 +/- 48 microg in sham to 516 +/- 160 microg after maternal adrenalectomy; inhibition of steroid production by metyrapone induced a further increase to 757 +/- 125 microg. Our data support the new concept of a negative role of glucocorticoids in fetal beta-cell development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / drug effects
  • Adrenal Glands / embryology
  • Adrenalectomy
  • Animals
  • Corticosterone / administration & dosage
  • Corticosterone / biosynthesis
  • Corticosterone / blood
  • Female
  • Fetal Blood / chemistry
  • Fetal Growth Retardation / etiology
  • Fetal Weight
  • Gestational Age
  • Glucocorticoids / physiology*
  • Insulin / analysis
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / embryology*
  • Metyrapone / pharmacology
  • Nutrition Disorders / blood
  • Nutrition Disorders / complications
  • Pancreas / chemistry
  • Pancreas / embryology
  • Pregnancy
  • Rats
  • Rats, Wistar

Substances

  • Glucocorticoids
  • Insulin
  • Corticosterone
  • Metyrapone