Induction of specific cytotoxic lymphocytes in mice vaccinated with Brucella abortus RB51

Infect Immun. 2001 Sep;69(9):5502-8. doi: 10.1128/IAI.69.9.5502-5508.2001.


A safe, more sensitive, nonradioactive, neutral red uptake assay was adopted to replace the traditional 51Cr release assay for detection of Brucella-specific cytotoxic T lymphocyte (CTL) activity. Our studies indicated that Brucella abortus strain RB51 vaccination of mice induced specific CTLs against both strain RB51- and strain 2308-infected J774.A1 macrophages but not against Listeria monocytogenes-infected J774.A1 cells. The antigen-specific cytotoxic activity was exerted by T lymphocytes but not by NK cells. CD3+ CD4+ T cells secreted the highest level of gamma interferon (IFN-gamma) and were able to exert a low but significant level of specific lysis of Brucella-infected macrophages. They also exerted a low level of nonspecific lysis of noninfected macrophages. In contrast, CD3+ CD8+ T cells secreted low levels of IFN-gamma but demonstrated high levels of specific lysis of Brucella-infected macrophages with no nonspecific lysis. These findings indicate that B. abortus strain RB51 vaccination of mice induces specific CTLs and suggest that CD3+ CD4+ and CD3+ CD8+ T cells play a synergistic role in the anti-Brucella activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Brucella Vaccine / immunology*
  • Brucella abortus / immunology*
  • Brucellosis / immunology
  • Brucellosis / prevention & control*
  • CD3 Complex / metabolism
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • T-Lymphocytes, Cytotoxic / immunology*
  • Vaccination


  • Brucella Vaccine
  • CD3 Complex

Associated data

  • GENBANK/L06299
  • GENBANK/L06302