Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomics

P R Jungblut; E C Müller; J Mattow; S H Kaufmann

doi:10.1128/IAI.69.9.5905-5907.2001

Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomics

Infect Immun. 2001 Sep;69(9):5905-7. doi: 10.1128/IAI.69.9.5905-5907.2001.

Authors

P R Jungblut¹, E C Müller, J Mattow, S H Kaufmann

Affiliation

¹ Core Facility for Protein Analysis, Max Planck Institute for Infection Biology, Berlin, Germany. jungblut@mpiib-mpg.de

Abstract

Genomics revealed the sequence of 3924 genes of the H37Rv strain of Mycobacterium tuberculosis. Proteomics complements genomics in showing which genes are really expressed, and here we show the expression of six genes not predicted by genomics, as proved by two-dimensional electrophoresis and matrix-assisted laser desorption ionization and nano-electrospray mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacterial Proteins / chemistry
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Electrophoresis, Gel, Two-Dimensional
Genome, Bacterial*
Humans
Molecular Sequence Data
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / metabolism
Open Reading Frames / genetics*
Proteome*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Bacterial Proteins
Proteome

Associated data

GENBANK/AX023830
PIR/A70573
SWISSPROT/14KD_MYCTU
SWISSPROT/Y525_MYCLE