Expression of the rabbit cytochrome P450 aromatase encoding gene uses alternative tissue-specific promoters

Eur J Biochem. 2001 Aug;268(16):4506-12. doi: 10.1046/j.1432-1327.2001.02375.x.

Abstract

The aim of the present study was to analyse the tissue-specific expression of various promoter-derived transcripts from the gene encoding rabbit aromatase cytochrome P450. A new promoter, named I.r, was identified, and promoters II and I.r were sequenced. Promoter I.r-derived transcripts were found in preovulatory granulosa cells, corpus luteum, placenta and adipose tissue. An alternative splice variant of this transcript was found with tissue-specific preference. Tissue-specific expression of promoter-derived variants was studied in the ovary before and after ovulation. While the level of promoter II-derived transcript decreased dramatically after ovulation, that of promoter I.r-derived transcript remained unchanged, indicating that promoter II and promoter I.r were not controlled by a single regulation system. The existence of this dual system of regulation suggests that the rabbit ovary could be a useful model to study the promoter-specific regulation of aromatase.

MeSH terms

  • Alternative Splicing
  • Animals
  • Aromatase / genetics*
  • Base Sequence
  • Female
  • Molecular Sequence Data
  • Organ Specificity
  • Promoter Regions, Genetic*
  • RNA, Messenger / analysis
  • Rabbits
  • TATA Box

Substances

  • RNA, Messenger
  • Aromatase