Genetic variation of in vitro cytolytic activity and in vivo rejection potential of non-immunized semi-syngeneic mice against a mouse lymphoma line

Int J Cancer. 1975 Jun 15;15(6):933-40. doi: 10.1002/ijc.2910150608.


Spleens of normal young mice of certain genotypes contain lymphocytes that can kill strain A-derived YAC-1 and some other in vitro-grown Moloney lymphoma lines in a 51Cr-release cytotoxic test. We have previously shown that mouse strains can be classified as high or low reactors in this test. F(1) hybrids between low- and high-reactive strains are high-reactive. In the present study, strain-A mice and eight different A F(1) hybrids were tested in parallel for their spleen-cell-mediated killing effect in vitro and their ability to reject graded numbers of YAC ascites or in vitro cultivated cells in vivo. There was a clear correlation between in vitro cytotoxicity and in vivo rejection in all tested genotypes. In segregating (A times C57Bl) times A backcross mice, in vivo rejection of YAC cells was H-2 linked. This is in line with the earlier backcross analysis of the in vitro cytotoxicity, suggesting a polygenic control with at least one H-2 linked factor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Crosses, Genetic
  • Cytotoxicity Tests, Immunologic
  • Genetic Linkage
  • Genetic Variation*
  • Genotype
  • Graft Rejection*
  • Histocompatibility
  • Hybridization, Genetic
  • Lymphocytes / immunology*
  • Lymphoma / immunology*
  • Mice
  • Mice, Inbred A
  • Mice, Inbred C57BL
  • Neoplasm Transplantation
  • Neoplasms, Experimental / immunology*
  • Spleen / immunology
  • Transplantation, Homologous