Stretch induces a growth factor in alveolar cells via protein kinase

Respir Physiol. 2001 Sep;127(2-3):105-11. doi: 10.1016/s0034-5687(01)00244-4.


Positive-pressure mechanical ventilation can injure the lung, causing edema and alveolar inflammation in a complication termed ventilator-induced lung injury (VILI). Cytokines such as interleukin-8 (IL-8) reportedly are important in this inflammatory response. On the other hand, hepatocyte growth factor (HGF) promotes regeneration of the lung, and delays pulmonary fibrosis. We postulated that cyclic stretch upregulates production and release of both of mediators. Human alveolar epithelial cells (A549) cultured on a silicoelastic membrane were tested for mRNA expression and release of IL-8 and HGF after cyclic stretch in vitro. Stretch induced mRNA expression and release of these mediators. The signaling pathway from cyclic stretch to release of IL-8 and HGF appeared to involve protein kinase C in the signal transduction pathway.

MeSH terms

  • Cells, Cultured
  • Enzyme Inhibitors / pharmacology
  • Epithelial Cells / cytology
  • Epithelial Cells / enzymology*
  • Gene Expression / physiology
  • Hepatocyte Growth Factor / genetics*
  • Humans
  • Interleukin-8 / genetics*
  • Peptides / pharmacology
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism*
  • Pulmonary Alveoli / cytology*
  • Pulmonary Edema / metabolism
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / physiology
  • Stress, Mechanical


  • Enzyme Inhibitors
  • Interleukin-8
  • Peptides
  • RNA, Messenger
  • Hepatocyte Growth Factor
  • Protein Kinase C