Detection of high-risk HPV types by the hybrid capture 2 test

J Med Virol. 2001 Sep;65(1):155-62.


A hybrid capture test that can be used to detect at least 13 high-risk HPV types (referred to collectively as HPV-HR) in cervical scrapes (Hybrid Capture 2 probe set B, HC2-B) was evaluated. The HC2-B test is accurate and highly reproducible and the results obtained show excellent agreement with those obtained by a multiplexed type-specific polymerase chain reaction (mts-PCR). An additional assay to identify a subset of HPV-HR types may improve specificity without compromising sensitivity in HC2-B positive specimens with a test result close to the cut-off value given by 1 pg/ml HPV DNA.

Publication types

  • Evaluation Study

MeSH terms

  • Cervix Uteri / virology
  • DNA, Viral / analysis
  • Female
  • Humans
  • Nucleic Acid Hybridization* / methods
  • Papillomaviridae / classification
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / virology
  • Polymerase Chain Reaction / methods
  • Polymorphism, Restriction Fragment Length
  • Reproducibility of Results
  • Tumor Virus Infections / diagnosis*
  • Tumor Virus Infections / virology


  • DNA, Viral