Characterization of a novel genital human papillomavirus by overlapping PCR: candHPV86 identified in cervicovaginal cells of a woman with cervical neoplasia

J Gen Virol. 2001 Sep;82(Pt 9):2035-2040. doi: 10.1099/0022-1317-82-9-2035.


A novel human papillomavirus (HPV), candHPV86, was cloned and characterized from cervicovaginal cells obtained from a 37-year-old Hispanic woman with cervical intraepithelial neoplasia grade 1 (CIN1) using an overlapping PCR technique. Primers were designed by phylogenetic alignment of closely related HPV genomes using the L1 fragment sequence amplified by GP5+/6+. The 7983 bp complete nucleotide sequence of the HPV genome was determined by sequence walking. A basic local alignment sequence tool (BLAST) homology search using the L1 open reading frame demonstrated that this HPV was most closely related to HPVHAN2294 (GenBank, AJ400628; 86% homology) and HPV84 (84% homology). candHPV86 was placed in the HPV genome homology group A3 by phylogenetic analyses. The overlapping PCR technique is applicable for characterizing the complete spectrum and variation of HPVs in a population.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Base Sequence
  • Cervix Uteri / virology*
  • Female
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics
  • Polymerase Chain Reaction*
  • Uterine Cervical Neoplasms / virology*
  • Vagina / virology*

Associated data

  • GENBANK/AF349909