Genetic Diversity and Molecular Epidemiology of Respiratory Syncytial Virus Over Four Consecutive Seasons in South Africa: Identification of New Subgroup A and B Genotypes

J Gen Virol. 2001 Sep;82(Pt 9):2117-2124. doi: 10.1099/0022-1317-82-9-2117.

Abstract

The molecular epidemiology of respiratory syncytial virus (RSV) was studied over four consecutive seasons (1997-2000) in a single tertiary hospital in South Africa: 225 isolates were subgrouped by RT-PCR and the resulting products sequenced. Subgroup A predominated in two seasons, while A and B co-circulated approximately equally in the other seasons. The nucleotide sequences of the C-terminal of the G-protein were compared to sequences representative of previously defined RSV genotypes. South African subgroup A and subgroup B isolates clustered into four and five genotypes respectively. One new subgroup A and three new subgroup B genotypes were identified. Different genotypes co-circulated in every season. Different circulation patterns were identified for group A and B isolates. Subgroup A revealed more variability and displacement of genotypes while subgroup B remained more consistent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Child, Preschool
  • Genetic Variation
  • Genotype
  • Humans
  • Infant
  • Infant, Newborn
  • Molecular Sequence Data
  • Respiratory Syncytial Virus, Human / classification*
  • Respiratory Syncytial Virus, Human / genetics
  • Seasons
  • South Africa

Associated data

  • GENBANK/AF348802
  • GENBANK/AF348803
  • GENBANK/AF348804
  • GENBANK/AF348805
  • GENBANK/AF348806
  • GENBANK/AF348807
  • GENBANK/AF348808
  • GENBANK/AF348809
  • GENBANK/AF348810
  • GENBANK/AF348811
  • GENBANK/AF348812
  • GENBANK/AF348813
  • GENBANK/AF348814
  • GENBANK/AF348815
  • GENBANK/AF348816
  • GENBANK/AF348817
  • GENBANK/AF348818
  • GENBANK/AF348819
  • GENBANK/AF348820
  • GENBANK/AF348821
  • GENBANK/AF348822
  • GENBANK/AF348823
  • GENBANK/AF348824
  • GENBANK/AF348825
  • GENBANK/AF348826