Novel vectors for co-expression of two proteins in E. coli

Biotechniques. 2001 Aug;31(2):322-3, 326-8. doi: 10.2144/01312st03.


Two new vectors, pAC28 and pEGST, for the co-expression of recombinant genes in E. coli were developed. This two-plasmid system allows for an efficient expression and purification of large amounts of protein-protein complexes formed in bacterial cells. We have utilized this new system to express and isolate a stable complex of two human proteins, hematopoietic cell tyrosine phosphatase (HePTP) and mitogen-activated proteins kinase Erk2. This approach is useful for biochemical and structural studies of protein-protein interactions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Technical Report

MeSH terms

  • Cloning, Molecular / methods
  • Escherichia coli / genetics*
  • Gene Expression Regulation, Bacterial
  • Genetic Vectors / genetics*
  • Humans
  • Mitogen-Activated Protein Kinase 1 / genetics
  • Plasmids / genetics*
  • Protein Tyrosine Phosphatases / genetics
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification*


  • Recombinant Proteins
  • Mitogen-Activated Protein Kinase 1
  • Protein Tyrosine Phosphatases