Sugar-responsible elements in the promoter of a gene for beta-amylase of sweet potato

Plant Mol Biol. 2001 Jul;46(5):627-37. doi: 10.1023/a:1010684908364.

Abstract

Expression of genes coding for sporamin and beta-amylase, the two most abundant proteins in storage roots of sweet potato, is coordinately inducible in atypical vegetative tissues by sugars. A sweet potato gene for beta-amylase (beta-Amy) with introns as well as a beta-Amy::GUS fusion gene composed of the beta-Amy promoter and the GUS coding sequence, both showed sugar-inducible expression in leaves of transgenic tobacco which occurred via a hexokinase-independent pathway. Analyses using various 5'-terminal and internal deletions of the beta-Amy promoter indicated that truncated promoters of beta-Amy containing a sequence between -901 and -820, relative to the transcription start site, and the basic promoter region can confer sugar-inducible expression. This 82 bp region contained the TGGACGG sequence that plays an essential role in the sugar-inducible expression of the truncated promoter of the sporamin gene. Deletion or base substitutions of this element in the truncated beta-Amy promoter abolished the sugar-inducible expression, the results suggesting that the TGGACGG element plays an important role in the coordinate induction of expression of genes for beta-amylase and sporamin by sugars.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carbohydrates / pharmacology*
  • DNA, Plant / drug effects
  • DNA, Plant / genetics
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Plant / drug effects
  • Glucuronidase / drug effects
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Molecular Sequence Data
  • Plants, Genetically Modified / genetics
  • Plants, Toxic
  • Promoter Regions, Genetic / genetics*
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Response Elements / genetics*
  • Response Elements / physiology
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • Solanaceae / enzymology
  • Solanaceae / genetics*
  • Tobacco / genetics
  • beta-Amylase / genetics*

Substances

  • Carbohydrates
  • DNA, Plant
  • Recombinant Fusion Proteins
  • beta-Amylase
  • Glucuronidase