Constituents of Cannabis sativa L. VIII: Possible biological application of a new method to separate cannabidiol and cannabichromene

J Pharm Sci. 1975 May;64(5):810-4. doi: 10.1002/jps.2600640517.


Synthetic and naturally occurring cannabidiol and cannabichromene were distinctly separated without derivation by GLC using a 6% OV-1 column; an artifact of cannabichromene, cannabicyclol, was separated from (minus)-delta9-trans-tetrahydrocanna-bivarin. This procedure is versatile and applicable for the quantitation of Cannabis containing both cannabidiol and cannabichromene. Biological interaction among (minus)-delta9-trans-tetrhydrocanabinol, cannabichromene, and other cannabinoids in natural Cannabis preparations can now be studied. In the phenyl methyl silicone polymer series, cannabidiol precedes ca-nabichromene on columns containing below a 50% phenyl-to-methyl ratio. Columns containing below a 50% phenyl-to-methyl ratio. Columns containing a 50:50 or greater ratio of phenyl to methyl reverse the separation order with cannabichromene preceding cannabidiol.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Benzopyrans / isolation & purification*
  • Cannabidiol / isolation & purification*
  • Cannabinoids
  • Cannabis / analysis*
  • Cannabis / isolation & purification*
  • Chromatography, Gas
  • Mass Spectrometry
  • Methods
  • Temperature


  • Benzopyrans
  • Cannabinoids
  • Cannabidiol
  • cannabichromene