MD-2 binds to bacterial lipopolysaccharide

J Endotoxin Res. 2000;6(6):489-91.

Abstract

Many LPS binding proteins have been described, but the exact nature of the LPS receptors that signal cells remains unclear. MD-2 is a molecule that is found in association with Toll-like receptor 4, which has been shown to be a receptor for LPS. We have produced human MD-2 in baculovirus and tested it for LPS binding. MD-2 binds the lipid A region of LPS without the need for LPS binding protein. These data suggest that MD-2 may be binding LPS as part of the TLR4 receptor complex.

MeSH terms

  • Animals
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism*
  • Baculoviridae / genetics
  • Cell Line
  • Cloning, Molecular
  • Drosophila Proteins*
  • Gene Expression
  • Humans
  • In Vitro Techniques
  • Lipid A / metabolism
  • Lipopolysaccharides / metabolism*
  • Lymphocyte Antigen 96
  • Membrane Glycoproteins / metabolism
  • Protein Binding
  • Receptors, Cell Surface / metabolism
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Toll-Like Receptor 4
  • Toll-Like Receptors

Substances

  • Antigens, Surface
  • Drosophila Proteins
  • LY96 protein, human
  • Lipid A
  • Lipopolysaccharides
  • Lymphocyte Antigen 96
  • Membrane Glycoproteins
  • Receptors, Cell Surface
  • Recombinant Proteins
  • TLR4 protein, human
  • Toll-Like Receptor 4
  • Toll-Like Receptors