Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11)

J Endotoxin Res. 2001;7(2):113-8.


A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [carbohydrate structure see text] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.

MeSH terms

  • Acinetobacter / chemistry*
  • Acinetobacter / classification
  • Animals
  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal / immunology
  • Blotting, Western
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cross Reactions
  • Epitopes
  • Humans
  • Magnetic Resonance Spectroscopy
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • Serotyping


  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Epitopes
  • O Antigens