Cloning of parsley flavone synthase I

Phytochemistry. 2001 Sep;58(1):43-6. doi: 10.1016/s0031-9422(01)00191-1.

Abstract

A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.

MeSH terms

  • Apigenin
  • Cloning, Molecular
  • DNA, Complementary
  • Flavanones*
  • Flavonoids / metabolism
  • Mixed Function Oxygenases / genetics*
  • Mixed Function Oxygenases / metabolism
  • Petroselinum / enzymology*
  • Plant Leaves / enzymology
  • Recombinant Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae / enzymology

Substances

  • DNA, Complementary
  • Flavanones
  • Flavonoids
  • Recombinant Proteins
  • Apigenin
  • Mixed Function Oxygenases
  • flavone synthase I
  • naringenin