The grp78 promoter of Neurospora crassa: constitutive, stress and differentiation-dependent protein-binding patterns

Curr Genet. 2001 Jul;39(5-6):319-26. doi: 10.1007/s002940100202.

Abstract

In order to characterize the transcriptional regulation of the grp78 (glucose-regulated protein 78 kDa) gene of Neurospora crassa, a 1.65-kb genomic fragment upstream of the protein-coding region was sequenced and analysed. A single transcription start point was mapped 160 nt upstream of the first codon. Several distinct protein-DNA binding sites were identified in the promoter region by non-radioactive scanning electrophoretic mobility shift analysis during growth, stress treatment and differentiation of conidia. A protein DNA binding complex induced by tunicamycin was linked to a promoter motif similar to the unfolded protein response element consensus of yeast. Another binding complex in differentiating aerial hyphae was found, which differs from the known cis-elements involved in conidiation-dependent gene expression.

MeSH terms

  • Base Sequence
  • Binding Sites
  • DNA, Fungal / metabolism
  • Fungal Proteins / genetics*
  • HSP70 Heat-Shock Proteins / genetics*
  • Molecular Sequence Data
  • Neurospora crassa / genetics*
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • Protein Binding
  • Protein Biosynthesis

Substances

  • DNA, Fungal
  • Fungal Proteins
  • HSP70 Heat-Shock Proteins
  • KAR2 protein, yeast