Identification of AXUD1, a novel human gene induced by AXIN1 and its reduced expression in human carcinomas of the lung, liver, colon and kidney

Oncogene. 2001 Aug 16;20(36):5062-6. doi: 10.1038/sj.onc.1204603.


Axin, an important regulator of beta-catenin, is frequently mutated in human hepatocellular carcinomas (HCCs), and transduction of the wild-type Axin gene (AXIN1) induces apoptosis in HCC cells as well as in colon cancer cells. To investigate the detailed biological function of Axin, we searched on a cDNA microarray for genes whose expression was altered by transfer of wild-type AXIN1 into colon-cancer cell line LoVo. Among the genes showing altered expression, we focused on one, termed AXUD1 (AXIN1 up-regulated), that revealed enhanced expression in response to exogenously expressed AXIN1 but not to LacZ, a control gene. The AXUD1 gene consists of five exons and encodes a transcript with an open reading frame of 1767 bp. A 3.2-kb transcript of AXUD1 was expressed in all human tissues examined, most abundantly in lung, placenta, skeletal muscle, pancreas and leukocyte. By radiation-hybrid mapping we assigned its chromosomal location at 3p22, a region where frequent loss of heterozygosity has been reported in lung, renal, prostate, breast and cervical cancers. AXUD1 was frequently down-regulated in lung, kidney, liver and colon cancers compared with their corresponding normal tissues, suggesting that AXUD1 may have a tumor-suppressor function in those organs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis Regulatory Proteins
  • Axin Protein
  • Base Sequence
  • COS Cells
  • Carcinoma / genetics
  • Carcinoma / metabolism*
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism
  • Humans
  • Kidney Neoplasms / genetics
  • Kidney Neoplasms / metabolism
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism
  • Molecular Sequence Data
  • Proteins / genetics
  • Proteins / metabolism*
  • Proteins / physiology*
  • RNA, Neoplasm / biosynthesis
  • Repressor Proteins*
  • Tissue Distribution
  • Transcription, Genetic
  • Tumor Cells, Cultured


  • AXIN1 protein, human
  • Apoptosis Regulatory Proteins
  • Axin Protein
  • CSRNP1 protein, human
  • Proteins
  • RNA, Neoplasm
  • Repressor Proteins

Associated data

  • GENBANK/AB053121