Metastin suppresses the motility and growth of CHO cells transfected with its receptor

Biochem Biophys Res Commun. 2001 Sep 7;286(5):958-63. doi: 10.1006/bbrc.2001.5470.


We recently reported having identified of the ligand for an orphan G-protein-coupled receptor, hOT7T175, as the gene product (68-121)-amide of the metastasis suppressor gene KiSS-1. We further showed that the ligand, which we named "metastin," inhibits chemotaxis and invasion of Chinese hamster ovary (CHO) cells transfected with hOT7T175 cDNA (CHO/h175) in vitro, and pulmonary metastasis of hOT7T175-transfected B16-BL6 melanomas in vivo. In the present study, we investigated the activity of metastin in CHO/h175 cells in greater detail. Metastin significantly suppressed motility in a chemotaxis assay and wound healing assay at 10-100 nM order concentrations. Two N-terminally truncated peptides, metastin(40-54) and metastin(45-54) inhibited the migration of CHO/h175 cells as potently as metastin itself. Metastin also inhibited the spreading, monolayer growth and colony formation in agar (0.8%) of CHO/h175 cells at 10-100 nM concentrations. These results indicate that metastin is a potent inhibitor of cell motility, leading to suppression of cell growth and antimetastatic activity, and suggest that low molecular chemical compounds could replace its activity as a novel antimetastatic agent.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • CHO Cells
  • Calcium / metabolism
  • Cell Division / drug effects
  • Cell Movement
  • Cells, Cultured
  • Chemotaxis / drug effects
  • Cricetinae
  • DNA, Complementary / metabolism
  • Dose-Response Relationship, Drug
  • Escherichia coli / metabolism
  • Humans
  • Kisspeptins
  • Ligands
  • Peptides / chemistry
  • Proteins / pharmacology*
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / metabolism
  • Receptors, G-Protein-Coupled
  • Receptors, Kisspeptin-1
  • Receptors, Neuropeptide*
  • Time Factors
  • Transfection
  • Tumor Suppressor Proteins
  • Wound Healing


  • Antineoplastic Agents
  • DNA, Complementary
  • KISS1 protein, human
  • KISS1R protein, human
  • Kisspeptins
  • Ligands
  • Peptides
  • Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Kisspeptin-1
  • Receptors, Neuropeptide
  • Tumor Suppressor Proteins
  • Calcium