Protection of radiation-induced protein damage by curcumin

Biophys Chem. 2001 Aug 30;92(1-2):119-26. doi: 10.1016/s0301-4622(01)00188-0.

Abstract

Free radical reactions of lysozyme (Lz), tryptophan and disulfides were studied with curcumin, a lipid-soluble antioxidant from turmeric, in aqueous solution using a pulse radiolysis technique. The binding of curcumin with lysozyme was confirmed using absorption, fluorescence and stopped-flow techniques. The free radicals of curcumin generated after repairing radicals of disulfides, lysozyme and tryptophan absorb at 500-510 nm. Implication of this in evaluating the antioxidant behavior of curcumin in protecting proteins is discussed.

MeSH terms

  • Algorithms
  • Anisotropy
  • Antioxidants / pharmacology
  • Curcumin / pharmacology*
  • Disulfides / chemistry
  • Free Radicals / chemistry
  • Muramidase / chemistry
  • Protein Binding
  • Proteins / drug effects*
  • Proteins / radiation effects*
  • Pulse Radiolysis
  • Radiation-Protective Agents / pharmacology*
  • Spectrometry, Fluorescence
  • Tryptophan / chemistry

Substances

  • Antioxidants
  • Disulfides
  • Free Radicals
  • Proteins
  • Radiation-Protective Agents
  • Tryptophan
  • Muramidase
  • Curcumin