Confirming yeast two-hybrid protein interactions using in vitro glutathione-S-transferase pulldowns

Methods Mol Biol. 2001;177:135-50. doi: 10.1385/1-59259-210-4:135.
No abstract available

MeSH terms

  • DNA-Directed RNA Polymerases / metabolism
  • Fungal Proteins / genetics*
  • Gene Library
  • Genes, Reporter
  • Genetic Techniques*
  • Glutathione Transferase / genetics*
  • Isopropyl Thiogalactoside / pharmacology
  • Methionine / metabolism
  • Protein Biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Saccharomyces cerevisiae / genetics*
  • Transcription, Genetic
  • Two-Hybrid System Techniques
  • Viral Proteins
  • beta-Galactosidase / antagonists & inhibitors

Substances

  • Fungal Proteins
  • Recombinant Fusion Proteins
  • Viral Proteins
  • Isopropyl Thiogalactoside
  • Methionine
  • Glutathione Transferase
  • bacteriophage T7 RNA polymerase
  • DNA-Directed RNA Polymerases
  • beta-Galactosidase