Antigen processing for MHC class I restricted presentation of exogenous influenza A virus nucleoprotein by B-lymphoblastoid cells

Clin Exp Immunol. 2001 Sep;125(3):423-31. doi: 10.1046/j.1365-2249.2001.01613.x.


In general, exogenous proteins are processed by antigen-presenting cells in the endosomes for major histocompatibility complex (MHC) class II presentation to CD4+ T cells, while proteins synthesized endogenously are processed in the cytoplasm for MHC class I presentation to CD8+ T cells. However, it is recognized that exogenous proteins can be processed for MHC class I presentation also, and evidence in favour of alternatives to the conventional MHC class I processing and presentation pathway is accumulating. Here, we show that exogenous recombinant influenza A virus nucleoprotein (rNP) is processed for MHC class I presentation to CD8+ cytotoxic T lymphocytes (CTL) by EBV-transformed, B-lymphoblastoid cell lines (B-LCL). Processing of rNP for HLA-B27-associated presentation seemed to follow the conventional MHC class I pathway predominantly, as presentation was diminished in the presence of lactacystin and brefeldin A, but was less sensitive to chloroquine and NH4Cl. HLA-B27-associated presentation was also observed using cells lacking a functional transporter associated with antigen processing, suggesting that alternative pathways may be exploited for processing of rNP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcysteine / analogs & derivatives*
  • Acetylcysteine / pharmacology
  • Antigen Presentation / drug effects
  • Antigen Presentation / immunology*
  • B-Lymphocytes / immunology*
  • Brefeldin A / pharmacology
  • Chloroquine / pharmacology
  • Endosomes / drug effects
  • Enzyme Inhibitors / pharmacology
  • Hematopoietic Stem Cells / immunology
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Influenza A virus / immunology*
  • Major Histocompatibility Complex*
  • Nucleocapsid Proteins
  • Nucleoproteins / immunology*
  • Peptide Fragments / immunology
  • RNA-Binding Proteins*
  • Viral Core Proteins / immunology*


  • Enzyme Inhibitors
  • Histocompatibility Antigens Class I
  • NP protein, Influenza A virus
  • Nucleocapsid Proteins
  • Nucleoproteins
  • Peptide Fragments
  • RNA-Binding Proteins
  • Viral Core Proteins
  • lactacystin
  • Brefeldin A
  • Chloroquine
  • Acetylcysteine