Scoring nuclear pleomorphism in breast cancer

Histopathology. 2001 Sep;39(3):259-65. doi: 10.1046/j.1365-2559.2001.01220.x.


Aims: Nuclear grade has equal weight with mitotic index and acinus formation in grading breast cancer, but criteria for its assessment are less well defined. This study examines consistency of nuclear grading in breast cancer and whether improved nuclear grading criteria are required.

Methods and results: Photographic prints of haematoxylin-eosin sections of 100 unselected symptomatic breast cancers were circulated to histopathologists who assigned each carcinoma a nuclear pleomorphism score on a linear analogue scale 0-100 (0-33 equating to nuclear pleomorphism grade 1, 34-66 to grade 2, and 67-100 to grade 3). Seventeen histopathologists completed the exercise, including 11 breast specialists. While kappa scores for the implied nuclear grades indicated 'moderate' or 'good' agreement between individuals and the group as a whole, seven pathologists allocated analogue scores significantly lower than the median score allocated by the group to each case, while five allocated significantly higher scores. The range was from analogue scores 11.3 units lower on average than the median (assigning 27% of carcinomas nuclear grade 1, 60% grade 2, and 13% grade 3) to scores 7.5 units higher on average than the median (assigning only 2% carcinomas nuclear grade 1, 46% grade 2, and 52% grade 3). Five of six non-specialists allocated scores significantly lower than the group medians but only two of 11 specialists did so (P=0.018).

Conclusions: Systematic differences between pathologists in scoring nuclear pleomorphism in breast cancer potentially contribute to differences in allocating overall grade and confirm the need for improved nuclear grading criteria. Specialists tend to allocate higher pleomorphism scores than non-specialists.

MeSH terms

  • Breast Neoplasms / pathology*
  • Breast Neoplasms / ultrastructure
  • Cell Nucleus / ultrastructure
  • Humans
  • Mitotic Index