UDP-sugar transporter implicated in glycosylation and processing of Notch

Nat Cell Biol. 2001 Sep;3(9):816-22. doi: 10.1038/ncb0901-816.

Abstract

Glycosylation modifies protein activities in various biological processes. Here, we report the functions of a novel UDP-sugar transporter (UST74C, an alternative name for Fringe connection (Frc)) localized to the Golgi apparatus in cellular signalling of Drosophila. Mutants in the frc gene exhibit phenotypes resembling wingless and Notch mutants. Both Fringe-dependent and Fringe-independent Notch pathways are affected, and both glycosylation and proteolytic maturation of Notch are defective in mutant larvae. The results suggest that changes in nucleotide-sugar levels can differently affect Wingless and two distinct aspects of Notch signalling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Crosses, Genetic
  • Drosophila Proteins
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Embryo, Nonmammalian / physiology
  • Female
  • Glycosylation
  • Green Fluorescent Proteins
  • Homozygote
  • Larva
  • Luminescent Proteins / analysis
  • Luminescent Proteins / genetics
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Mutagenesis
  • Ovary / embryology
  • Receptors, Notch
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Uridine Diphosphate Sugars / metabolism*
  • Wings, Animal / embryology

Substances

  • Drosophila Proteins
  • Luminescent Proteins
  • Membrane Proteins
  • N protein, Drosophila
  • Receptors, Notch
  • Uridine Diphosphate Sugars
  • Green Fluorescent Proteins

Associated data

  • GENBANK/AB062677