Induction of T lymphocyte responses to dengue virus by a candidate tetravalent live attenuated dengue virus vaccine

Vaccine. 2001 Sep 14;19(32):4694-9. doi: 10.1016/s0264-410x(01)00236-5.


Development of a safe and immunogenic tetravalent dengue virus (DV) vaccine has been designated as a priority by the World Health Organization. We characterized the T cell response to DV induced by a candidate live attenuated tetravalent DV vaccine as part of a phase I study. Proliferation and cytotoxic T lymphocyte (CTL) responses to multiple DV serotypes were detected in six of six and four of four subjects studied, respectively. Proliferation responses were higher to DV serotypes 1 and 3 than to serotypes 2 and 4. CTL responses were higher to DV serotypes 2 and 3 than to serotype 1, and included serotype cross-reactive responses. Production of interferon-gamma, but not IL-4, was observed in response to DV stimulation. This candidate vaccine is immunogenic for both CD4+ and CD8+ T lymphocytes. However, T cell responses to the four DV serotypes were not equivalent, suggesting that the vaccine could be further optimized.

Publication types

  • Clinical Trial
  • Clinical Trial, Phase I
  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / biosynthesis
  • Antibodies, Viral / immunology
  • B-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / immunology*
  • Culture Media, Conditioned / chemistry
  • Cytokines / metabolism
  • Cytotoxicity, Immunologic
  • Dengue Virus / immunology*
  • Genetic Variation
  • Humans
  • Immunologic Memory
  • Interferon-gamma / metabolism
  • Interleukin-4 / metabolism
  • Lymphocyte Activation
  • Neutralization Tests
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / metabolism
  • Vaccination
  • Vaccines, Attenuated / immunology
  • Vaccinia virus / genetics
  • Vaccinia virus / immunology
  • Viral Vaccines / immunology*


  • Antibodies, Viral
  • Culture Media, Conditioned
  • Cytokines
  • Vaccines, Attenuated
  • Viral Vaccines
  • Interleukin-4
  • Interferon-gamma