Real-time PCR for detection and quantitation of hepatitis B virus DNA

J Med Virol. 2001 Oct;65(2):250-6. doi: 10.1002/jmv.2027.


A sensitive and reproducible real-time PCR assay based on TaqMan technology was developed for the detection and quantitation of hepatitis B virus (HBV) DNA in serum, and compared with an "in-house" qualitative PCR assay. HBV DNA was measured in 125 serum samples from 76 hepatitis B patients, consisting of 22 patients with an acute infection, 20 patients with a previous history of hepatitis B infection, and 34 patients with a chronic hepatitis B. Four patients with a chronic infection were treated with either an IFN-alpha monotherapy or a combination of IFN-alpha and lamivudine. Twenty-nine sera from healthy individuals and non-hepatitis B patients served as negative controls. The assay was validated by using a 10-fold dilution series of the World Virological Quality Control (VQC) sample containing 3.73 x 10(7) genome equivalents per ml. The detection limit for the real-time PCR was 3.73 x 10(2) genome equivalents per ml (geq/ml), while it was 3.73 x 10(3) geq/ml for the in-house PCR. The real-time PCR assay had an 8-logarithm dynamic range spanning from 10(2) to 10(10) geq/ml. In clinical serum samples, the real-time PCR and the in-house PCR detected HBV DNA in 81% (101/125) and 66% (83/125) of samples, respectively. HBV DNA was not detected among the negative controls by either of these assays. In conclusion, real-time PCR is a sensitive, specific, and a reproducible approach for the detection and quantitation of HBV DNA in clinical serum samples, useful also for monitoring the efficacy of antiviral treatment.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antiviral Agents / therapeutic use
  • Computers
  • DNA, Viral / blood*
  • Drug Therapy, Combination
  • Follow-Up Studies
  • Hepatitis B / blood
  • Hepatitis B / drug therapy
  • Hepatitis B / virology*
  • Hepatitis B virus / genetics
  • Hepatitis B virus / isolation & purification*
  • Hepatitis B, Chronic / virology
  • Humans
  • Interferon-alpha / therapeutic use
  • Lamivudine / therapeutic use
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / standards
  • Predictive Value of Tests
  • Sensitivity and Specificity


  • Antiviral Agents
  • DNA, Viral
  • Interferon-alpha
  • Lamivudine