Chemical synthesis of oligoribonucleotides containing 2-aminopurine: substrates for the investigation of ribozyme function

J A Doudna; J W Szostak; A Rich; N Usman

doi:10.1021/jo00308a003

Chemical synthesis of oligoribonucleotides containing 2-aminopurine: substrates for the investigation of ribozyme function

J Org Chem. 1990 Oct 12;55(21):5547-9. doi: 10.1021/jo00308a003.

Authors

J A Doudna¹, J W Szostak, A Rich, N Usman

Collaborator

A Rich²

Affiliations

¹ Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
² MA Inst Technol, Cambridge

PMID: 11540922
DOI: 10.1021/jo00308a003

Abstract

The chemical synthesis of a fully protected ribonucleoside phosphoramidite, containing 2-aminopurine as the base component, and its incorporation into short oligoribonucleotides as substrates for an engineered ribozyme from Tetrahymena is described.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

2-Aminopurine / analogs & derivatives
2-Aminopurine / chemistry*
Animals
Directed Molecular Evolution
Guanosine / chemistry
Molecular Biology / methods
Molecular Structure
Oligoribonucleotides / chemical synthesis*
RNA, Catalytic / chemistry*
RNA, Catalytic / metabolism
Ribonucleosides / chemistry*
Substrate Specificity
Tetrahymena / enzymology*

Substances

Oligoribonucleotides
RNA, Catalytic
Ribonucleosides
Guanosine
2-Aminopurine