Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity

J Tissue Cult Methods. 1992;14(2):51-7. doi: 10.1007/BF01404744.

Abstract

High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bioreactors*
  • Cell Count
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Line
  • Cricetinae
  • Equipment Design
  • Kidney / cytology
  • Microspheres
  • Rotation*
  • Weightlessness Simulation*