Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona

J Bacteriol. 2001 Oct;183(19):5725-32. doi: 10.1128/JB.183.19.5725-5732.2001.


This study describes the characterization of the recently described Salmonella genomic island 1 (SGI1) (D. A. Boyd, G. A. Peters, L.-K. Ng, and M. R. Mulvey, FEMS Microbiol. Lett. 189:285-291, 2000), which harbors the genes associated with the ACSSuT phenotype in a Canadian isolate of Salmonella enterica serovar Typhimurium DT104. A 43-kb region has been completely sequenced and found to contain 44 predicted open reading frames (ORFs) which comprised approximately 87% of the total sequence. Fifteen ORFs did not show any significant homology to known gene sequences. A number of ORFs show significant homology to plasmid-related genes, suggesting, at least in part, a plasmid origin for the SGI1, although some with homology to phage-related genes were identified. The SGI1 was identified in a number of multidrug-resistant DT120 and S. enterica serovar Agona strains with similar antibiotic-resistant phenotypes. The G+C content suggests a potential mosaic structure for the SGI1. Emergence of the SGI1 in serovar Agona strains is discussed.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacteriophage Typing
  • Base Sequence
  • Drug Resistance, Microbial / genetics*
  • Drug Resistance, Multiple / genetics
  • Genome, Bacterial*
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Plasmids / genetics
  • Salmonella Phages / genetics
  • Salmonella typhimurium / classification
  • Salmonella typhimurium / drug effects
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / virology
  • Sequence Analysis, DNA
  • Serotyping


  • Anti-Bacterial Agents

Associated data

  • GENBANK/AF261825