Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes

J Biol Chem. 2001 Nov 23;276(47):44003-11. doi: 10.1074/jbc.M105669200. Epub 2001 Sep 6.


A human protein kinase, p53-related protein kinase (PRPK), was cloned from an interleukin-2-activated cytotoxic T-cell subtraction library. PRPK appears to be a homologue of a growth-related yeast serine/threonine protein kinase, YGR262c. However, a complementation assay using YGR262c-disrupted yeast indicated that PRPK is not functionally identical to the yeast enzyme. PRPK expression was observed in interleukin-2-activated cytotoxic T-cells, some human epithelial tumor cell lines, and the testes. The intrinsic transcriptional activity of p53 was up-regulated by a transient transfection of PRPK to COS-7 cells. PRPK was shown to bind to p53 and to phosphorylate p53 at Ser-15. These results indicate that PRPK may play an important role in the cell cycle and cell apoptosis through phosphorylation of p53.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Interleukin-2 / pharmacology*
  • Intracellular Signaling Peptides and Proteins
  • Lymphocyte Activation
  • Male
  • Molecular Sequence Data
  • Phosphorylation
  • Phylogeny
  • Polymerase Chain Reaction
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • Protein Serine-Threonine Kinases
  • Sequence Homology, Amino Acid
  • T-Lymphocytes, Cytotoxic / drug effects*
  • T-Lymphocytes, Cytotoxic / metabolism
  • Testis / cytology
  • Testis / drug effects*
  • Testis / metabolism
  • Transcription, Genetic
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / metabolism


  • DNA Primers
  • DNA, Complementary
  • Interleukin-2
  • Intracellular Signaling Peptides and Proteins
  • Tumor Suppressor Protein p53
  • Protein Kinases
  • Protein Serine-Threonine Kinases
  • TP53RK protein, human

Associated data

  • GENBANK/AB017505
  • GENBANK/AB028045