Characterization of a sea bream (Sparus aurata) thyroid hormone receptor-beta clone expressed during embryonic and larval development

Gen Comp Endocrinol. 2001 Jul;123(1):80-9. doi: 10.1006/gcen.2001.7649.


A clone encoding thyroid hormone receptor-beta (TR-beta) was isolated from a sea bream (Sparus aurata) ovary cDNA library. Sea bream (sb)TR-beta is closely related to its counterparts from other vertebrates and, like them, preferentially binds T3 rather than T4. However, the putative sbTR-beta protein contains a nine-amino-acid insert that is also present in the corresponding proteins from flounder and salmon but absent in TR-betas from zebrafish and terrestrial vertebrates. Semiquantitative RT-PCR analysis showed that sbTR-beta transcripts begin to accumulate during gastrulation and increase markedly in quantity up to the period around hatch (ca. 40 h postfertilization) before declining slightly. In adult tissues, TR-beta mRNA was present in approximately equal quantities in heart, intestine, brain, kidney, skeletal muscle, liver, and gill. The significance of the relatively strong expression of TR-beta during sea bream embryogenesis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification
  • Electrophoresis, Polyacrylamide Gel
  • Embryonic Development*
  • Female
  • Gene Expression*
  • Gene Library
  • Larva / growth & development*
  • Molecular Sequence Data
  • Ovary / chemistry
  • Receptors, Thyroid Hormone / genetics*
  • Receptors, Thyroid Hormone / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sea Bream* / genetics
  • Sequence Alignment
  • Thyroxine / metabolism
  • Triiodothyronine / metabolism


  • DNA, Complementary
  • Receptors, Thyroid Hormone
  • Triiodothyronine
  • Thyroxine