Protection against ultraviolet B- and C-induced DNA damage and skin carcinogenesis by the flowers of Prunus persica extract

Mutat Res. 2001 Sep 20;496(1-2):47-59. doi: 10.1016/s1383-5718(01)00218-2.


The ethanol extract of the flowers of Prunus persica (Ku-35) (50-200 microg/ml) was found to inhibit UVB- as well as UVC-induced DNA damage measured by the COMET assay in the skin fibroblast cell (NIH/3T3). In addition, Ku-35 inhibited UVB- or UVC-induced lipid peroxidation, especially against UVB-induced peroxidation at higher than 10 microg/ml. We also evaluated the protective effect of Ku-35 against UVB-induced non-melanoma skin cancer in mice. Ku-35 was applied topically before UVB exposure, and its effects on tumor incidence (% of mice with tumors) and tumor multiplicity (number of tumors per mouse) were evaluated. The application of Ku-35 clearly resulted in a delay of tumor development compared to the control. In tumor incidence, 100% mice in the control group and the low dose treatment of Ku-35 had tumors, whereas 94.1% of the mice had tumors after the high dose treatment of Ku-35 at the end of experiment (28 weeks). In tumor multiplicity, low and high treatments of Ku-35 resulted in 25.9 and 53.9% reduction at the end of the experiment (P<0.05, one-way analysis of variance (ANOVA)). The present data indicate that Ku-35 protects against photogenotoxicity in NIH/3T3 fibroblasts. The possible action mechanism of Ku-35 may be through its anti-oxidant activity without pro-oxidant effect. Ku-35 can also show a delay of tumor development against UVB-induced skin carcinogenesis. These results suggest that Ku-35 extract may be useful for protecting UV-induced DNA damage and carcinogenesis when topically applied.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Anticarcinogenic Agents / pharmacology*
  • Comet Assay
  • DNA / radiation effects
  • DNA Damage / drug effects*
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Radiation
  • Female
  • Image Processing, Computer-Assisted
  • Mice
  • Mice, Nude
  • Neoplasms, Radiation-Induced / pathology
  • Neoplasms, Radiation-Induced / prevention & control*
  • Plant Extracts / pharmacology*
  • Radiation-Protective Agents / pharmacology*
  • Rosales / chemistry*
  • Skin Neoplasms / pathology
  • Skin Neoplasms / prevention & control*
  • Ultraviolet Rays / adverse effects*


  • Anticarcinogenic Agents
  • Plant Extracts
  • Radiation-Protective Agents
  • DNA