Tissue-specific and isoform-specific changes in MCT1 and MCT4 in heart and soleus muscle during a 1-yr period

Am J Physiol Endocrinol Metab. 2001 Oct;281(4):E749-56. doi: 10.1152/ajpendo.2001.281.4.E749.

Abstract

We examined the postnatal changes (days 10, 36, 84, 160, 365) of monocarboxylate transporters (MCT)1 and MCT4 in rat heart and soleus muscle. In the heart, MCT1 protein and mRNA remained unaltered from day 10 until 1 yr of age. Both MCT4 protein and mRNA in the heart were detected at 10 days of age, but the MCT4 protein and transcript were not detected thereafter. In the soleus muscle, MCT1 protein (+38%) and mRNA (+136%) increased during the first 84 days and remained stable until 1 yr of age. In contrast, soleus MCT4 protein decreased by 90% over the course of 1 yr, with the most rapid decrease (-60%) occurring by day 84 (P < 0.05). At the same time, MCT4 mRNA was increased by 74% from days 10 to 84 (P < 0.05), remaining stable thereafter. In conclusion, developmental changes in MCT transport proteins are tissue specific and isoform specific. Furthermore, it appears that MCT1 expression in the heart and MCT1 and MCT4 expression in the soleus are regulated by pretranslational processes, whereas posttranscriptional processes regulate MCT4 expression in the soleus muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Body Weight
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Gene Expression Regulation, Developmental*
  • Heart / growth & development*
  • Male
  • Membrane Proteins / genetics
  • Monocarboxylic Acid Transporters
  • Muscle Development*
  • Muscle Proteins*
  • Muscle, Skeletal / growth & development*
  • Muscle, Skeletal / metabolism
  • Myocardium / metabolism
  • Organ Size
  • Protein Isoforms / genetics
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Transcription, Genetic

Substances

  • Carrier Proteins
  • Membrane Proteins
  • Monocarboxylic Acid Transporters
  • Muscle Proteins
  • Protein Isoforms
  • RNA, Messenger
  • Slc16a3 protein, rat