Drosophila mitochondrial transcription factor A: characterization of its cDNA and expression pattern during development

Biochem Biophys Res Commun. 2001 Sep 21;287(2):474-83. doi: 10.1006/bbrc.2001.5528.

Abstract

We cloned a cDNA for Drosophila mitochondrial transcription factor A (D-mtTFA) and characterized the recombinant protein. In Drosophila Kc cells, D-mtTFA was localized in the mitochondria, but not in the nucleus. By repetitive precipitation with His-tag and PCR amplification, the consensus nucleotide sequence for D-mtTFA-binding was determined to be 5'-TTATC/G. The binding sequence was found to be clustered in the A + T region of mitochondrial DNA which is suggested to be a replication origin and promoter region for light strand and heavy strand. We found a DNA replication-related element (DRE)-like sequence located upstream of the transcription initiation site of the D-mtTFA gene and obtained results indicating that DRE-binding factor (DREF) can bind to the DRE-like sequence of the D-mtTFA gene. The data suggest that transcription of the D-mtTFA gene is under control of the DRE/DREF regulatory system. Based on these results, the functions of D-mtTFA were discussed in relation to mitochondrial biogenesis of Drosophila melanogaster.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Division
  • Cells, Cultured
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary / analysis
  • DNA-Binding Proteins*
  • Drosophila melanogaster / genetics
  • Gene Expression Regulation*
  • Humans
  • Mitochondria / genetics*
  • Mitochondrial Proteins*
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Proteins / classification
  • Nuclear Proteins / genetics*
  • Subcellular Fractions
  • Transcription Factors / classification
  • Transcription Factors / genetics*

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Mitochondrial Proteins
  • Nuclear Proteins
  • TFAM protein, human
  • Transcription Factors
  • mitochondrial transcription factor A