Defining the repeating elements in the cysteine-rich region (CRR) of the CD18 integrin beta 2 subunit

FEBS Lett. 2001 Sep 7;505(1):27-30. doi: 10.1016/s0014-5793(01)02778-8.

Abstract

The cysteine-rich region (CRR) of the integrin beta subunits is organised into four repeating elements. By expression of a panel of truncated beta 2 subunits, and CRR segments fused to the C-terminal end of a CD4 soluble fragment, the segment required for the expression of two monoclonal antibody conformational epitopes was determined. This segment, E482-Q574, contains 16 cysteines representing two repeating units. We have thus defined the CRR unit motif of 'xC---C---C---CxCxxCxC---Cx', where 'x' represents a single residue, and '---' represents a stretch of four to 14 residues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • CD18 Antigens / genetics
  • CD18 Antigens / immunology*
  • CD4 Antigens / genetics
  • CD4 Antigens / metabolism
  • COS Cells
  • Cysteine
  • Epitopes / chemistry*
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology*
  • Repetitive Sequences, Amino Acid*

Substances

  • Antibodies, Monoclonal
  • CD18 Antigens
  • CD4 Antigens
  • Epitopes
  • Recombinant Fusion Proteins
  • Cysteine