Differences in proton leak kinetics, but not in UCP3 protein content, in subsarcolemmal and intermyofibrillar skeletal muscle mitochondria from fed and fasted rats

FEBS Lett. 2001 Sep 7;505(1):53-6. doi: 10.1016/s0014-5793(01)02772-7.

Abstract

We have investigated the effect of 24-h fasting on basal proton leak and uncoupling protein (UCP) 3 expression at the protein level in subsarcolemmal and intermyofibrillar skeletal muscle mitochondria. In fed rats, the two mitochondrial populations displayed different proton leak, but the same protein content of UCP3. In addition, 24-h fasting, both at 24 and 29 degrees C, induced an increase in proton leak only in subsarcolemmal mitochondria, while UCP3 content increased in both the populations. From the present data, it appears that UCP3 does not control the basal proton leak of skeletal muscle mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / metabolism*
  • Fasting
  • Ion Channels
  • Kinetics
  • Male
  • Mitochondria, Muscle / metabolism*
  • Mitochondrial Proteins
  • Muscle, Skeletal / metabolism*
  • Myofibrils / metabolism*
  • Protons
  • Rats
  • Rats, Wistar
  • Sarcolemma / metabolism*
  • Temperature
  • Uncoupling Protein 3

Substances

  • Carrier Proteins
  • Ion Channels
  • Mitochondrial Proteins
  • Protons
  • Ucp3 protein, rat
  • Uncoupling Protein 3