High cyclin E and low p27/Kip1 expressions are potentially poor prognostic factors in lung adenocarcinoma patients

Lung Cancer. 2001 Oct;34(1):59-65. doi: 10.1016/s0169-5002(01)00211-2.


Cyclin E is an important regulator of entry into the S phase of the cell cycle. p27/Kip1 (p27) binds to cyclin E/Cdk2 complex and negatively regulates cell proliferation. We immunohistochemically examined the expression of cyclin E and p27 in 98 cases of resected lung adenocarcinoma to evaluate the prognostic significance of cyclin E and p27. Cyclin E was expressed in 16 cases (16%), and p27 was expressed in 41 cases (42%). Using Kaplan-Meier survival analysis, patients with cyclin E positive (P=0.0017) and p27 negative (P=0.011), both individually and in combination (P<0.0001), had a worse prognosis. We also analyzed the relationship of these findings to clinicopathological parameters, which revealed that cyclin E-positive, p27-negative cases had a higher Ki67 expression (P=0.012) and a higher rate of lymph node metastasis (P=0.0078) than other groups. Our results suggested that cyclin E over expression, in association with p27 reduction in particular, may potentially be a poor prognostic factor in lung adenocarcinoma patients. However, to verify the prognostic significance of these factors, a multivariate analysis of a larger number of patients should be undertaken.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology*
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / biosynthesis*
  • Cell Cycle Proteins / analysis
  • Cell Cycle Proteins / biosynthesis*
  • Cyclin E / analysis
  • Cyclin E / biosynthesis*
  • Cyclin-Dependent Kinase Inhibitor p27
  • Enzyme Inhibitors / analysis*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Immunohistochemistry
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / pathology*
  • Male
  • Middle Aged
  • Prognosis
  • Survival Analysis
  • Tumor Suppressor Proteins / analysis
  • Tumor Suppressor Proteins / biosynthesis*


  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • Cyclin E
  • Enzyme Inhibitors
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27