The sequence of a 15 769 bp segment of Pichia anomala identifies the SEC61 and FBP1 genes and five new open reading frames

Yeast. 2001 Sep 30;18(13):1187-95. doi: 10.1002/yea.766.

Abstract

We have determined the sequence of a 15 769 bp DNA segment of Pichia anomala. The sequence contains seven complete open reading frames (ORFs) longer than 100 amino acids and a putative tRNA gene. Two of the ORFs code for the well-characterized genes SEC61 (which codes for the core subunit of the ER translocation complex) and FBP1 (encoding fructose-1,6-bisphosphatase). A gene coding for a protein similar to S. cerevisiae YDL054c was found between the two genes. These three genes show a different organization (intermingled triples) in three yeast species: Saccharomyces cerevisiae, Candida albicans and P. anomala. Two out of the four remaining ORFs show weak homology with different proteins from other species and the other two show non-significant similarity with previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession No. AJ306295.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • DNA, Fungal
  • Fructose-Bisphosphatase / chemistry
  • Fructose-Bisphosphatase / genetics*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Open Reading Frames / genetics*
  • Pichia / enzymology
  • Pichia / genetics*
  • SEC Translocation Channels
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • DNA, Fungal
  • Membrane Proteins
  • SEC Translocation Channels
  • Fructose-Bisphosphatase

Associated data

  • GENBANK/AJ306295