Enhanced inhibition of HIV-1 replication in macrophages by antisense oligonucleotides, ribozymes and acyclic nucleoside phosphonate analogs delivered in pH-sensitive liposomes

Nucleosides Nucleotides Nucleic Acids. Apr-Jul 2001;20(4-7):515-23. doi: 10.1081/NCN-100002327.

Abstract

An antisense oligodeoxynucleotide against the human immunodeficiency virus type 1 (HIV-1) Rev response element, a ribozyme complementary to the HIV-1 5'-LTR, and the reverse transcriptase inhibitors 9-(2-phosphonylmethoxyethyl) adenine (PMEA) and (R)-9-(2-phosphonylmethoxypropyl)-adenine (PMPA) inhibited virus replication in monocyte-derived macrophages more effectively when delivered in pH-sensitive liposomes compared to the free drugs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenine / administration & dosage*
  • Adenine / analogs & derivatives*
  • Adenine / pharmacokinetics
  • Anti-HIV Agents / administration & dosage*
  • Anti-HIV Agents / pharmacokinetics
  • Genes, env / genetics
  • HIV-1 / drug effects*
  • HIV-1 / physiology
  • Humans
  • Hydrogen-Ion Concentration
  • Liposomes
  • Macrophages / metabolism
  • Macrophages / virology*
  • Oligonucleotides, Antisense / administration & dosage*
  • Oligonucleotides, Antisense / genetics
  • Organophosphonates*
  • Organophosphorus Compounds / administration & dosage*
  • Organophosphorus Compounds / pharmacokinetics
  • RNA, Catalytic / administration & dosage*
  • Tenofovir
  • Thionucleotides / administration & dosage
  • Virus Replication / drug effects*

Substances

  • Anti-HIV Agents
  • Liposomes
  • Oligonucleotides, Antisense
  • Organophosphonates
  • Organophosphorus Compounds
  • RNA, Catalytic
  • Thionucleotides
  • adefovir
  • Tenofovir
  • Adenine