DNA sequence recognition by NF kappa B p50 homodimer: strict and obscure recognition sites in the binding sequence

Nucleosides Nucleotides Nucleic Acids. Apr-Jul 2001;20(4-7):669-72. doi: 10.1081/NCN-100002347.

Abstract

5-Formyl- and 5-(formylmethyl)-2'-deoxyuridines are introduced into a kappa B site instead of thymidine(s) in order to understand target sequence specificity of NF kappa B. It was found that one thymidine in the kappa B site is particularly important for the sequence specific recognition by NF kappa B.

MeSH terms

  • Base Sequence
  • Binding Sites
  • Dimerization
  • Models, Molecular
  • NF-kappa B / chemistry
  • NF-kappa B / genetics*
  • NF-kappa B / metabolism*
  • NF-kappa B p50 Subunit
  • Oligonucleotides / chemical synthesis
  • Oligonucleotides / metabolism
  • Substrate Specificity
  • Thymidine / metabolism

Substances

  • NF-kappa B
  • NF-kappa B p50 Subunit
  • Oligonucleotides
  • Thymidine