Synthesis and evaluation of alpha-LNA

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):825-8. doi: 10.1081/NCN-100002438.

Abstract

Three different synthetic routes to the alpha-configured LNA thymine monomer starting from D-allose or D-arabinose were investigated. The introduction of one or four alpha-LNA monomers into alpha-DNA had a destabilizing effect on the duplexes. However, a fully modified alpha-LNA sequence displayed strong recognition of complementary RNA, but no transition with DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabinose / chemistry
  • DNA / chemistry
  • Glucose / chemistry
  • Nucleic Acid Conformation
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • RNA / chemistry
  • Thymine / analogs & derivatives
  • Thymine / chemistry

Substances

  • Oligonucleotides
  • allose
  • RNA
  • DNA
  • Arabinose
  • Glucose
  • Thymine