Identification of Peroxisomal Membrane Proteins of Saccharomyces Cerevisiae by Mass Spectrometry

Electrophoresis. 2001 Aug;22(14):2955-68. doi: 10.1002/1522-2683(200108)22:14<2955::AID-ELPS2955>3.0.CO;2-U.

Abstract

The identification of peroxisomal membrane proteins is very important to understand the import mechanisms of substrates and proteins into these organelles and the pathogenesis of human peroxisomal disorders like the Zellweger Syndrom. Peroxisomal membrane proteins were identified after separation by gel electrophoresis, tryptic digestion and mass spectrometric analysis. Using matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and nanoliquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), it was possible to identify 45 proteins of isolated yeast peroxisomal membranes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Membrane Proteins / analysis*
  • Molecular Sequence Data
  • Peroxisomes / chemistry*
  • Proteome*
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae Proteins / analysis*
  • Spectrometry, Mass, Electrospray Ionization*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*

Substances

  • Membrane Proteins
  • Proteome
  • Saccharomyces cerevisiae Proteins