Nasal administration of cholera toxin (CT) suppresses clinical signs of experimental autoimmune encephalomyelitis (EAE)

Vaccine. 2001 Oct 12;20(1-2):134-9. doi: 10.1016/s0264-410x(01)00278-x.

Abstract

Cholera toxin (CT), a major enterotoxin produced by Vibrio cholerae, elicits mucosal adjuvant activities by inducing antigen-specific CD4+ T cells secreting T helper type 2 (Th2) cytokines. Experimental autoimmune encephalomyelitis (EAE) is induced by Th1 cells specific for myelin-derived antigens. We induced EAE in C57BL/6 mice with myelin oligodendrocyte glycoprotein (MOG) 35-55 and CT was nasally administered as an immunomodulator on day 7 following MOG challenge. Clinical severity in the CT-treated mice was milder when compared to PBS-treated mice, while the levels of expression of interleukin (IL)-12 and interferon (IFN)-gamma in the central nervous system (CNS) of CT-treated mice were lower than PBS-treated mice. Thus, nasal administration of the mucosal immunomodulator CT ameliorated the severity of EAE, which was associated with the suppression of Th1 cell responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage
  • Adjuvants, Immunologic / pharmacology
  • Adjuvants, Immunologic / therapeutic use*
  • Administration, Intranasal
  • Amino Acid Sequence
  • Animals
  • Autoimmune Diseases / immunology
  • Autoimmune Diseases / therapy*
  • Central Nervous System / immunology
  • Central Nervous System / metabolism
  • Cholera Toxin / administration & dosage
  • Cholera Toxin / immunology
  • Cholera Toxin / pharmacology
  • Cholera Toxin / therapeutic use*
  • Drug Evaluation, Preclinical
  • Encephalomyelitis, Autoimmune, Experimental / immunology
  • Encephalomyelitis, Autoimmune, Experimental / therapy*
  • Female
  • Glycoproteins / immunology
  • Glycoproteins / toxicity
  • Humans
  • Interferon-gamma / metabolism
  • Interleukin-12 / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments / immunology
  • Peptide Fragments / toxicity
  • Severity of Illness Index
  • Spleen / immunology
  • Th1 Cells / immunology
  • Th2 Cells / immunology*
  • Th2 Cells / metabolism

Substances

  • Adjuvants, Immunologic
  • Glycoproteins
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • myelin oligodendrocyte glycoprotein (35-55)
  • Interleukin-12
  • Interferon-gamma
  • Cholera Toxin