Dehydroepiandrosterone (DHEA) stimulates glucose uptake in rat adipocytes: activation of phospholipase D

Comp Biochem Physiol B Biochem Mol Biol. 2001 Oct;130(3):359-64. doi: 10.1016/s1096-4959(01)00444-4.

Abstract

We examined the effect of dehydroepiandrosterone (DHEA) on glucose uptake and phospholipase D (PLD) activation in rat adipocytes. DHEA (1 microM) provoked a twofold increase in [3H]2-deoxyglucose (DG) uptake for 30 min. Incorporation of [3H]glycerol into diacylglycerol was increased 150% above basal level for 20 min after stimulation with 1 microM DHEA. DHEA increased PLD activity, measured by the incorporation into [3H]phosphatidylethanol in [3H]palmitate labelled rat adipocytes, or by [3H]choline release in [methyl-(3)H]choline labeled rat adipocytes. Our results suggest that DHEA stimulates glucose uptake with activation of PLD in rat adipocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / drug effects*
  • Adipocytes / enzymology
  • Adipocytes / metabolism
  • Animals
  • Biological Transport / drug effects
  • Chromatography, Thin Layer
  • Dehydroepiandrosterone / pharmacology*
  • Deoxyglucose / metabolism
  • Diglycerides / biosynthesis
  • Diglycerides / metabolism
  • Enzyme Activation / drug effects
  • Glucose / metabolism*
  • Phospholipase D / metabolism*
  • Rats
  • Time Factors

Substances

  • Diglycerides
  • Dehydroepiandrosterone
  • Deoxyglucose
  • Phospholipase D
  • Glucose