Light-emitting diode treatment reverses the effect of TTX on cytochrome oxidase in neurons

Neuroreport. 2001 Oct 8;12(14):3033-7. doi: 10.1097/00001756-200110080-00011.

Abstract

Light close to and in the near-infrared range has documented benefits for promoting wound healing in human and animals. However, mechanisms of its action on cells are poorly understood. We hypothesized that light treatment with a light-emitting diode array at 670 nm (LED) is therapeutic in stimulating cellular events involving increases in cytochrome oxidase activity. LED was administered to cultured primary neurons whose voltage-dependent sodium channels were blocked by tetrodotoxin. The down-regulation of cytochrome oxidase activity by TTX was reverted to control levels by LED. LED alone also up-regulated enzyme activity. Thus, the results are consistent with our hypothesis that LED has a stimulating effect on cytochrome oxidase in neurons, even when they have been functionally silenced by TTX.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology
  • Aging / radiation effects
  • Animals
  • Animals, Newborn
  • Cells, Cultured / drug effects
  • Cells, Cultured / enzymology
  • Cells, Cultured / radiation effects*
  • Dose-Response Relationship, Drug
  • Down-Regulation / physiology
  • Down-Regulation / radiation effects*
  • Electron Transport Complex IV / metabolism
  • Electron Transport Complex IV / radiation effects*
  • Infrared Rays / therapeutic use*
  • Nerve Degeneration / chemically induced
  • Nerve Degeneration / enzymology
  • Nerve Degeneration / prevention & control
  • Neurons / drug effects
  • Neurons / enzymology
  • Neurons / radiation effects*
  • Photic Stimulation / instrumentation*
  • Photic Stimulation / methods
  • Rats
  • Sodium Channel Blockers
  • Sodium Channels / metabolism
  • Tetrodotoxin / pharmacology
  • Visual Cortex / drug effects
  • Visual Cortex / enzymology
  • Visual Cortex / radiation effects
  • Wound Healing / physiology
  • Wound Healing / radiation effects*

Substances

  • Sodium Channel Blockers
  • Sodium Channels
  • Tetrodotoxin
  • Electron Transport Complex IV