Abstract
High-fat diet alters apo E-dependent processing of beta-amyloid precursor protein. Here we have evaluated the effects of dietary fat on brain apo E mRNA in Zucker lean and obese rats. After approximately 2 months on a high-fat diet, there was significant up-regulation of brain apo E mRNA in the Zucker lean rat in parallel with weight gain. Densitometric quantification revealed a 17% increase in apo E mRNA in the brains of lean rats fed high-fat diet compared with those of lean rats fed rat chow. No significant difference in brain apo E mRNA of Zucker obese rats fed different diets was found. These results suggest that dietary fat alters brain apo E levels, which may be regulated, in part, through the leptin receptor.
MeSH terms
-
Alzheimer Disease / diet therapy
-
Alzheimer Disease / genetics
-
Alzheimer Disease / metabolism
-
Amyloid beta-Peptides / biosynthesis
-
Amyloid beta-Peptides / genetics
-
Animals
-
Apolipoproteins E / genetics*
-
Appetite Regulation / genetics*
-
Brain / cytology
-
Brain / metabolism*
-
Brain Chemistry / genetics
-
Carrier Proteins / metabolism
-
Dietary Fats / metabolism*
-
Eating / genetics
-
Food, Formulated / adverse effects
-
Leptin / metabolism
-
Obesity / genetics
-
Obesity / metabolism
-
Obesity / physiopathology
-
RNA, Messenger / metabolism*
-
Rats
-
Rats, Zucker / anatomy & histology
-
Rats, Zucker / metabolism*
-
Receptors, Cell Surface*
-
Receptors, Leptin
-
Up-Regulation / genetics*
-
Weight Gain / genetics
Substances
-
Amyloid beta-Peptides
-
Apolipoproteins E
-
Carrier Proteins
-
Dietary Fats
-
Leptin
-
RNA, Messenger
-
Receptors, Cell Surface
-
Receptors, Leptin