Ovarian carcinoma cells synthesize both chondroitin sulfate and heparan sulfate cell surface proteoglycans that mediate cell adhesion to interstitial matrix

J Cell Biochem. 2001 Aug 1-9;83(2):259-70. doi: 10.1002/jcb.1230.

Abstract

Metastatic ovarian carcinoma metastasizes by intra-peritoneal, non-hematogenous dissemination. The adhesion of the ovarian carcinoma cells to extracellular matrix components, such as types I and III collagen and cellular fibronectin, is essential for intra-peritoneal dissemination. The purpose of this study was to determine whether cell surface proteoglycans (a class of matrix receptors) are produced by ovarian carcinoma cells, and whether these proteoglycans have a role in the adhesion of ovarian carcinoma cells to types I and III collagen and fibronectin. Proteoglycans were metabolically labeled for biochemical studies. Both phosphatidylinositol-anchored and integral membrane-type cell surface proteoglycans were found to be present on the SK-OV-3 and NIH:OVCAR-3 cell lines. Three proteoglycan populations of differing hydrodynamic size were detected in both SK-OV-3 and NIH:OVCAR-3 cells. Digestions with heparitinase and chondroitinase ABC showed that cell surface proteoglycans of SK-OV-3 cells had higher proportion of chondroitin sulfate proteoglycans (75:25 of chondroitin sulfate:heparan sulfate ratio), while NIH:OVCAR-3 cells had higher proportion of heparan sulfate proteoglycans (10:90 of chondroitin sulfate:heparan sulfate ratio). RT-PCR indicated the synthesis of a unique assortment of syndecans, glypicans, and CD44 by the two cell lines. In adhesion assays performed on matrix-coated titer plates both cell lines adhered to types I and III collagen and cellular fibronectin, and cell adhesion was inhibited by preincubation of the matrix with heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, or chondroitin glycosaminoglycans. Treatment of the cells with heparitinase, chondroitinase ABC, or methylumbelliferyl xyloside also interfered with adhesion confirming the role of both heparan sulfate and chondroitin sulfate cell surface proteoglycans as matrix receptors on ovarian carcinoma cells.

MeSH terms

  • Binding Sites
  • Binding, Competitive
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Chondroitin Sulfates / metabolism*
  • Chondroitin Sulfates / pharmacology
  • Collagen Type I / metabolism
  • Collagen Type III / metabolism
  • Female
  • Fibronectins / metabolism
  • Glycosaminoglycans / metabolism*
  • Heparitin Sulfate / metabolism*
  • Heparitin Sulfate / pharmacology
  • Humans
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Ovarian Neoplasms / metabolism*
  • Proteoglycans / metabolism
  • Proteoglycans / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tumor Cells, Cultured / metabolism

Substances

  • Collagen Type I
  • Collagen Type III
  • Fibronectins
  • Glycosaminoglycans
  • Membrane Glycoproteins
  • Proteoglycans
  • RNA, Messenger
  • Chondroitin Sulfates
  • Heparitin Sulfate