Longitudinal variability of human immunodeficiency virus type 1 RNA viral load measurements by nucleic acid sequence-based amplification and NucliSens assays in a large multicenter study

J Clin Microbiol. 2001 Oct;39(10):3760-3. doi: 10.1128/JCM.39.10.3760-3763.2001.

Abstract

Human immunodeficiency virus type 1 (HIV-1) RNA measurements were evaluated within an externally controlled multilaboratory program. Three external standards (1.5 x 10(3) to 1.5 x 10(6) copies/ml) were included in 814 assay runs by four laboratories. Results indicate that HIV-1 RNA levels can be measured with a precision equal to that of the pre-highly active antiretroviral therapy era (standard deviations, +/-0.16 to 0.25 log10 units).

Publication types

  • Evaluation Study
  • Multicenter Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • HIV-1 / physiology*
  • Laboratories
  • Nucleic Acid Amplification Techniques / methods
  • Nucleic Acid Amplification Techniques / standards*
  • Quality Control
  • RNA, Viral / blood*
  • Reference Values
  • Self-Sustained Sequence Replication / methods
  • Self-Sustained Sequence Replication / standards*
  • Viral Load
  • Virology

Substances

  • RNA, Viral