Extrusion of actin-positive strands from Hep-2 and Int 407 cells caused by outer membrane preparations of enteropathogenic Escherichia coil and specific attachment of wild type bacteria to the strands

Can J Microbiol. 2001 Aug;47(8):727-34.

Abstract

Enteropathogenic Escherichia coli (EPEC) causes persistent infantile diarrhoea. This nontoxigenic E. coli exhibits a complicated pathogenic mechanism in which its outer membrane proteins and type III secretory proteins damage intestinal epithelium and cause diarrhoea. In accordance with this, our previous study using HEp-2 cells demonstrated cytopathic effects caused by cell-free outer membrane preparations of EPEC. In this study, we report the extrusion of actin-positive strands from HEp-2 and Int 407 cells when treated with outer membrane preparations. An interesting observation of this work, perhaps relevant to the characteristic localized three-dimensional colony formation of EPEC, is the attachment of a wild type EPEC strain to these actin-positive strands.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / metabolism*
  • Actin Cytoskeleton / microbiology*
  • Actinin / metabolism
  • Bacterial Adhesion*
  • Bacterial Outer Membrane Proteins / pharmacology*
  • Cell Line / drug effects
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Humans

Substances

  • Bacterial Outer Membrane Proteins
  • Actinin