The replication of certain Coxsackie B virus strains in CHO cells

J Virol Methods. 2001 Nov;98(2):161-5. doi: 10.1016/s0166-0934(01)00363-9.


Cell surface molecules that can act as viral receptors may exert an important selective pressure on RNA viral quasi-species. Coxsackie-Adenovirus Receptor and Decay Accelerating Factor (DAF, CD55) have been identified as receptors for Coxsackie B virus. In studies of viral replication using different strains of Coxsackievirus serotype 4 (CBV-4), it was found that despite lack of expression of these cell surface molecules on Chinese Hamster Ovary (CHO) cells and despite their common use as negative controls in Coxsackie B virus receptor assays, two strains were able to replicate, one (V89-4557) without cytopathic effect (CPE), and the other (T318) with strong CPE. A weak signal was obtained using antibodies against enterovirus, visualized with FITC-conjugated antibodies, when the Coxsackievirus B4 strain V89-4557 was inoculated on CHO cells compared to no signal with the non-replicating Coxsackievirus B4 strain VD2921, indicating some degree of binding of the former to the cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / metabolism*
  • CD55 Antigens / metabolism
  • CHO Cells
  • Cells, Cultured
  • Cricetinae
  • Cricetulus
  • Cytopathogenic Effect, Viral
  • Enterovirus B, Human / classification
  • Enterovirus B, Human / growth & development*
  • Enterovirus B, Human / metabolism*
  • Enterovirus B, Human / physiology
  • Fluorescein-5-isothiocyanate / metabolism
  • HeLa Cells
  • Humans
  • Hyaluronan Receptors / metabolism
  • Intercellular Adhesion Molecule-1 / metabolism


  • Antibodies, Monoclonal
  • CD55 Antigens
  • Hyaluronan Receptors
  • Intercellular Adhesion Molecule-1
  • Fluorescein-5-isothiocyanate