Performance of Pseudomonas CFC-selective Medium in the Fish Storage Ecosystems

J Microbiol Methods. 2001 Nov;47(2):243-7. doi: 10.1016/s0167-7012(01)00313-x.

Abstract

Pseudomonas agar base supplemented with cephaloridine, fucidin, and cetrimide (CFC) was used to count Pseudomonas populations on fish. Both Enterobacteriaceae and Shewanella putrefaciens were able to grow on the CFC medium. Evaluation of the performance of CFC-selective for pseudomonads medium, on fish samples stored aerobically and under a modified atmosphere at 0, 10 and 20 degrees C was tested. The selectivity of the medium was affected by storage temperatures and the type of packaging of the fish samples. The selectivity of the medium diminished as the population increased and for samples stored at high temperature (20 degrees C) or under modified atmospheres. When designing adequate selectivity of a medium, interfering organisms should be taken into account, especially when the background flora tends to be more robust than the organisms to be counted or detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cephaloridine / pharmacology*
  • Cetrimonium
  • Cetrimonium Compounds / pharmacology*
  • Colony Count, Microbial
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • Ecosystem*
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / growth & development
  • Enterobacteriaceae / isolation & purification
  • Environment, Controlled
  • Fishes / microbiology*
  • Food Handling / methods
  • Food Microbiology
  • Food Preservation / methods
  • Meat / microbiology
  • Pseudomonas / drug effects*
  • Pseudomonas / growth & development*
  • Pseudomonas / isolation & purification
  • Shewanella / drug effects
  • Shewanella / growth & development
  • Shewanella / isolation & purification
  • Substrate Specificity
  • Temperature

Substances

  • Cetrimonium Compounds
  • Culture Media
  • Cephaloridine
  • Cetrimonium